PhD - Biochemistry
MSc - Biochemistry
BSc - Chemistry
- BCEM 431 - Proteins And Proteomics
- BCEM 551 - Structural Biology
- CMMB 530A - Honours Research Project in Cellular, Molecular and Microbial Biology
- CMMB 507.95 - Advanced Topics in Cellular, Molecular and Microbial Biology (Honours Thesis)
- CMMB 530B - Honours Research Project in Cellular, Molecular and Microbial Biology
Research and teaching
Our research has been focused on a number of different proteins and peptides that play a role in infectious and inflammatory diseases or in cancer. Moreover we are actively involved in various clinical metabolomics research programs in Calgary.
Calcium-binding regulatory proteins are studied through site-directed mutagenesis, calorimetry experiments, spectroscopic studies and NMR structure determination. Together these experimental techniques provide very detailed information about protein structure, their metal ion binding sites, and they can define the interaction surfaces for target protein binding. Our work centers on the regulatory calcium binding protein calmodulin and structurally related calcium-binding proteins, such as the calcium-integrin binding proteins. At the moment we are studying various protein-protein interactions of mammalian calcium-binding proteins that play a role in the innate immune response or in cancer metastasis. Moreover calcium-regulatory proteins that play a major role in the plant stress response are also under study.
Host-defense peptides form an integral part of the innate immune response of humans and many other organisms. We are particularly interested in several tryptophan-rich antimicrobial peptides found in neutrophils or in digests of regular food proteins. Our work is aimed at elucidating the mechanism of action of various host defense peptides and proteins. Our studies rely on NMR structure determination of membrane-bound peptides, which are analyzed in combination with microcalorimetry and membrane leakage studies. Moreover we are interested in other host-defense proteins such as lysozyme, liocalin and lactoferrin. We are also studying the anticancer activities of various peptides.
Metabolomics is an emerging area of NMR spectroscopy and mass spectrometry research, where the full complement of metabolites that is present in a specific biological compartment is determined. By quantitatively analyzing the changes that occur in the metabolite profiles in serum, urine or other biofluid samples obtained from patients, diseases such as cancer or infectious diseases, might be detected earlier or better managed. Apart from the potential clinical applications of metabolomics research it also provides a powerful tool to compare antibiotic-resistant and -sensitive microorganisms or develop improved microbiological strains for biotechnology applications. Finally, metabolomics technologies, can also be used to study the effects of exercise, diet and nutritional supplements (e.g. vitamins) on the overall health in animal models and in humans.
- Ishida H, Jensen KV, Woodman AG, Hyndman ME and Vogel HJ (2017) “The calcium-dependent switch helix of L-plastin regulates actin-bundling” Sci. Rep. 7: 40662.
- Paul S, Ishida H, Nguyen LT, Liu Z and Vogel HJ (2017) “The solution structure of a free-standing acyl-carrier protein involved in the biosynthesis of cyclic lipopeptide antibiotics” Protein Sci 28: 946-959.
- Arias M and Vogel HJ (2017) “Fluorescence spectroscopy methods to study membrane perturbations by antimicrobial host defense peptides” Meth. Mol. Biol. 1548: 141-157.
- Arias M, Prenner EM, and Vogel HJ (2017) “Calorimetry methods to study membrane binding and perturbations by antimicrobial host defense peptides” Meth. Mol. Biol. 1548: 119-140.
- Choi D, Park E, Jung E, Seong YJ, Yoo J, Lee E, Hong M, Lee S, Ishida H, Burford J, Peti-Peterdi J, Adams RH, Srikanth S, Gwack Y, Chen CS, Vogel HJ, Koh CJ, Wong AK, and Hong YK (2017) “Laminar flow downregulates Notch activity to promote lymphatic sprouting” J. Clin. Invest. doi:10.1172/JCI87442.
- Patterson M, Vogel HJ and Prenner EJ (2017) “The effect of repeated lateral compression and expansions mimicking blinking on selected tear film polar lipid biofilms” Biochim. Biophys. Acta – Biomembranes 1859: 319-330.
- Paul S, Banerjee S, Vogel HJ (2017) “Ligand binding specificity of the Escherichia coli periplasmic histidine binding protein, HisJ” Protein Sci. 26: 268-279.
- Arias M, Hilchie A, Haney EF, Bolscher JGM, Hyndman ME, Hancock RE, Vogel HJ (2017) “Anticancer activities of bovine and human lactoferricin-derived peptides” Biochem. Cell Biol. 95: 91-98
- Nguyen LT, Vogel HJ. (2016) “Staphylokinase has distinct modes of interaction with antimicrobial peptides, modulating its plasminogen-activation properties” Sci. Rep. 6: 31817.
- Ishida H, Nguyen LT, Gopal R, Aizawa T, Vogel HJ. (2016) “Overexpression of antimicrobial, anticancer, and transmembrane peptides in Escherichia coli through a calmodulin-peptide fusion system” J. Am. Chem. Soc. 138: 11318-11326.
- Arias M, Hoffarth ER, Ishida H, Aramini JM, Vogel HJ (2016) “Recombinant expression, antimicrobial activity and mechanism of action of tritrpticin analogs containing fluoro-tryptophan residues” Biochim. Biophys. Acta 1858: 1012-1023.
- Lau CK, Krewulak KD, Vogel HJ (2016) “Bacterial ferrous iron transport: the Feo system” FEMS Microbiol. Rev. 40: 273-298.
- Patterson M, Vogel HJ, Prenner EJ (2016) “Biophysical characterization of monofilm model systems composed of selected tear film phospholipids” Biochim. Biophys. Acta 1858: 403-414.
- Banerjee S, Paul S, Nguyen LT, Chu BC, Vogel HJ (2016) “FecB, a periplasmic ferric-citrate transporter from E. coli, can bind different forms of ferric-citrate as well as a wide variety of metal-free and metal-loaded tricarboxylic acids” Metallomics 8: 125-133.
- Liu Z, García-Díaz B, Catacchio B, Chiancone E, Vogel HJ (2015) “Protecting Gram-negative bacterial cell envelopes from human lysozyme: Interactions with Ivy inhibitor proteins from Escherichia coli and Pseudomonas aeruginosa” Biochim. Biophys. Acta 1848: 3032-3046.
- Arias, M., Jensen, K.V., Storey, D.G. and Vogel, H.J. (2015) “Hydroxy-tryptophan containing derivatives of tritrpticin; modification of antimicrobial activity and membrane interactions” Biochim. Biophys. Acta – Biomembranes 1848: 277-288.
- Chu, B.C., Otten, R., Mulder F.A.A. and Vogel, H.J. (2014) “The solution structure, binding properties, and dynamics of the 34 kDa bacterial siderophore binding protein FepB” J. Biol. Chem. 289: 29219-29234.
- Arias, M., Nguyen, L.T., Kuczynski, A.M., Lejon, T. and Vogel, H.J. (2014) “Position-dependent influence of the three Trp residues on the membrane activity of the antimicrobial peptide tritrpticin” Antibiotics 3: 595-616.
- Ulke-Lemee, A., Ishida, H., Chappellaz, M., Vogel, H.J. and MacDonald, J.A. (2014) "Two domains of the smoothelin-like 1 protein bind apo- and calcium-calmodulin independently" Biochim. Biophys. Acta – Proteins and Proteomics 1844: 1580-1590.
- Ishida, H., Garcia-Herrera, A., and Vogel, H.J. (2014) "The periplasmic domain of Escherichia coli outer membrane protein A can undergo a localized temperature dependent structural transition" Biochim. Biophys. Acta – Biomembranes 1838: 3014-3024.
- Arias, M., MacDonald, L., Haney, E.F., Nazmi, K., Bolscher, J.M. and Vogel, H.J. (2014) "Bovine and human lactoferricin peptides: chimeras and new cyclic analogs" Biometals 27: 935-948.
Metabolomics Research (selected)
- Falegan OS, Ball MW, Shaykhutdinov RA, Pierorazio PM, Farshidfar F, Vogel HJ, Allaf ME, Hyndman ME (2017) “Urine and serum metabolomics may distinguish between stages of renal cell carcinoma” Metabolites 7(1): E6.
- Falegan OS, Vogel HJ, Hittel DS, Koch LG, Britton SL, Hepple RT and Shearer J (2017) “High aerobic capacity mitigates changes in the plasma metabolomics profile associated with aging” J. Proteome Res. 16: 798-805.
- Dowlatabadi R, Farshidfar F, Zare Z, Hemdani MP, Rabiei M, Khoshayand MR and Vogel HJ (2017) “Detection of adulteration in Iranian saffron samples by 1H NMR spectroscopy and multivariate data analysis techniques” Metabolomics 13: 19.
- Farshidfar F, Weljie AM, Kopciuk KA, Hilsden R, McGregor SE, Buie WD, MacLean A, Vogel HJ, Bathe OF (2016). “A validated metabolomic signature for colorectal cancer: exploration of the clinical value of metabolomics.” Br. J. Cancer 115: 848-857.
- Sarfaraz MO, Myers RP, Coffin CS, Gao ZH, Shaheen AA, Crotty PM, Zhang P, Vogel HJ, Weljie AM (2016) “A quantitative metabolomics profiling approach for the noninvasive assessment of liver histology in patients with chronic hepatitis C.” Clin. Transl. Med. 5: 33.
- Hoerr V, Duggan GE, Zbytnuik L, Poon KK, Große C, Neugebauer U, Methling K, Löffler B, Vogel HJ (2016) “Characterization and prediction of the mechanism of action of antibiotics through NMR metabolomics.” BMC Microbiol. 16: 82.
- Klein MS, Newell C, Bomhof MR, Reimer RA, Hittel DS, Rho JM, Vogel HJ, Shearer J (2016) “Metabolomic Modeling To Monitor Host Responsiveness to Gut Microbiota Manipulation in the BTBR(T+tf/j) Mouse.” J. Proteome Res.15: 1143-1150.
- Paul HA, Bomhof MR, Vogel HJ, Reimer RA (2016) “Diet-induced changes in maternal gut microbiota and metabolomic profiles influence programming of offspring obesity risk in rats.”Sci. Rep. 6: 20683.
- Mickiewicz B, Shin SY, Pozzi A, Vogel HJ, Clark AL. (2016) “Serum Metabolite Profiles Are Altered by Erlotinib Treatment and the Integrin α1-Null Genotype but Not by Post-Traumatic Osteoarthritis.” J. Proteome Res.15: 815-25.
- Mickiewicz B, Thompson GC, Blackwood J, Jenne CN, Winston BW, Vogel HJ, Joffe AR (2015) “Development of metabolic and inflammatory mediator biomarker phenotyping for early diagnosis and triage of pediatric sepsis.”Crit. Care 19: 320.
- Mickiewicz B, Kelly JJ, Ludwig TE, Weljie AM, Wiley JP, Schmidt TA, Vogel HJ (2015) “Metabolic analysis of knee synovial fluid as a potential diagnostic approach for osteoarthritis." J. Orthop. Res. 33: 1631-1638.
- Mickiewicz B, Tam P, Jenne CN, Leger C, Wong J, Winston BW, Doig C, Kubes P, Vogel HJ (2015) Integration of metabolic and inflammatory mediator profiles as a potential prognostic approach for septic shock in the intensive care unit.” Crit. Care19: 11.
- Mickiewicz, B., Heard B.J., Chau J.K., Chung, M., Hart, D.A., Shrive N.G., Frank, C.B. and Vogel, H.J. (2015) “Metabolic profiling of synovial fluid in a unilateral ovine model of anterior cruciate ligament reconstruction of the knee” J. Orthop. Res. 33: 71-77.
- Mickiewicz, B., Villemaire, M.L., Sandercock L.E., Jirik, F.R., and Vogel, H.J. (2014) “Metabolic changes associated with selenium deficiency in mice” Biometals 27: 1137-1147.
- Klein, M.S., Connors, K.E., Shearer, J., Vogel, H.J. and Hittel, D.S. (2014) “Metabolomics reveals the sex specific effects of the SORT1 low-density lipoprotein cholesterol locus in healthy young adults” J. Proteome Res. 13: 5063-5070.
- Palmnäs, M.S.A., Cowan, T.E., Bomhof M.R., Su, J., Reimer, R.A., Vogel, H.J., Hittel, D.S., Shearer, J. (2014) “Aspartame consumption differentially affects gut microbiota – host metabolic interactions in the diet-induced obese rat” PloS One 9(10): e109841.
- Cowan, T.E., Palmnas, M.S.A., Yang, J., Bomhof, M.R., Ardell, K., Reimer, R.A., Vogel, H.J., and Shearer, J. (2014) "Chronic coffee consumption in the diet-induced obese rat: impact on gut microbiota and serum metabolomics" J. Nutr. Biochem. 25: 489-495.
- 2001 - AHFMR Scientist Renewal (2001-2006)
- 2003 - Immigrant of Distinction Award, in recognition of outstanding professional accomplishments (Calgary Immigrant Society)
- 2004 - Outstanding Achievements in Supervision Award, Faculty of Graduate Studies Univ. of Calgary
- 2005 - Killam Resident Fellowship, University of Calgary
- 2006 - AHFMR Scientist Renewal (2006-2013)
- 2006 - Finalist - Outstanding leadership in Science ASTech Awards
- 2009 - Roche Diagnostics Award from the Canadian Society for Biochemistry, Molecular and Cell Biology
- 2011 - Molecular Cancer Epidemiology Award
- 2012 - Fellow of the Royal Society of Canada